How Changes in Nitrogen Levels Impact Metabolites and Physiological Features of Hypericum androsaemum

Background: The manipulation of mineral nutrition may affect secondary metabolite production and thus determine the nutritional value food/medicinal plants. Objectives: present study investigated impact nitrogen supply on metabolites physiological parameters Hypericum androsaemum plants grown in sand culture. Methods: Four-week were irrigated by full-strength Hoagland solution (control), nitrate was reduced to 1/3, no provided for 14 days. Results: growth shoots inhibited limitation, but root stimulated. Soluble proteins water content significantly affected deficiency. Antioxidative enzyme activities (ascorbate peroxidase (APX) superoxide dismutase (SOD)) increased due Treatment with NO3- deficiency caused an increase total soluble phenols phenylalanine ammonia-lyase (PAL) activity, while flavonoid mainly elevated At level specific metabolites, positively hypericin pseudohypericin decreased hyperforin content. expression putative gene biosynthesis (hyp-1) up-regulated response Conclusions: results demonstrated that H. plant antioxidant system, valuable phenols, metabolites.